Cytosolic Hsp70 and Hsp40 chaperones enable the biogenesis of mitochondrial β-barrel proteins
نویسندگان
چکیده
منابع مشابه
Roles of cytosolic Hsp70 and Hsp40 molecular chaperones in post-translational translocation of presecretory proteins into the endoplasmic reticulum.
Hsp70 molecular chaperones and their co-chaperones work together in various cellular compartments to guide the folding of proteins and to aid the translocation of proteins across membranes. Hsp70s stimulate protein folding by binding exposed hydrophobic sequences thereby preventing irreversible aggregation. Hsp40s stimulate the ATPase activity of Hsp70s and target unfolded proteins to Hsp70s. G...
متن کاملThe Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas.
Mutations at the APM1 and APM2 loci in the green alga Chlamydomonas reinhardtii confer resistance to phosphorothioamidate and dinitroaniline herbicides. Genetic interactions between apm1 and apm2 mutations suggest an interaction between the gene products. We identified the APM1 and APM2 genes using a map-based cloning strategy. Genomic DNA fragments containing only the DNJ1 gene encoding a type...
متن کاملBiogenesis and folding of β-barrel membrane proteins
β-barrel membrane proteins are composed of multiple antiparallel β-strands and form pores in the outer membranes of endosymbiotic organelles like mitochondria and the evolutionary related Gramnegative bacteria. These β-barrel channels are crucial for signal transduction, metabolite transport, and protein translocation. How are β-barrel membrane proteins assembled into the outer membrane? After ...
متن کاملMitochondrial β-Barrel Proteins, an Exclusive Club?
Mitochondria, chloroplasts, and Gram-negative bacteria have transporter proteins in their outer membranes called β-barrel proteins. The mechanism of integration of these proteins into the outer membrane of bacteria has been partly elucidated, including the identification of a C-terminal motif consisting of the terminal amino acid phenylala-nine and additional hydrophobic residues close to the C...
متن کاملCharacterization of the targeting signal in mitochondrial β-barrel proteins
Mitochondrial β-barrel proteins are synthesized on cytosolic ribosomes and must be specifically targeted to the organelle before their integration into the mitochondrial outer membrane. The signal that assures such precise targeting and its recognition by the organelle remained obscure. In the present study we show that a specialized β-hairpin motif is this long searched for signal. We demonstr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2018
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.201712029